So sánh lac operon và trp operon năm 2024

Regulates production of enzyme B-galactosidase which is needed to break down lactose. It consists of a cluster of 3 genes under the control of one promoter and one operator. When lactose levels are low the LacI repressor protein binds to lac operator, covering part of the promoter region which blocks transcription of mRNA (does not allow RNA polymerase to bind).

The Repressor protein binds to the operator when lactose levels are low. When lactose levels are high, the LacI repressor needs to be removed so lactose itself acts as an inducer or signal molecule. Lactose binds to LacI protein, changing its shape and it falls off operator allowing transcription of B-galactosidase to continue; High levels of lactose induce the Operon.

Regulates the production of the amino acid tryptophan. Consists of a cluster of 5 genes under the control of one promoter. When tryptophan levels are high (diet), it acts as a corepressor and binds to trp repressor protein-altering its shape so that it can bind to trp operator blocking transcription of mRNA.

Repressor-tryptophan complex binds to the operator when trp levels are high. When trp levels are low, the shape of the tryptophan corepressor complex changes (due to loss of tryptophan) and falls off the operator allowing transcription of tryptophan to continue; High levels of tryptophan represses the Operon.

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Operon is a special gene alignment in prokaryotes. In one operon, it aligns all the genes needed for a specific function. This organization allows one single promoter to activate, deactivate, and regulate all the genes participating in one particular function. Due to this nature, operon is called the functional unit of prokaryotic gene expression. Lac operon and Trp operon are two operons found in E.coli bacterial genome, and in many other bacteria. These operons control different functions. Operon is the functional unit of prokaryotic gene expression.

Lac Operon

Lac operon is the cluster of genes responsible for lactose transportation and metabolism in E.coli bacteria. The operon has one promoter region and genes lac Z, lac Y, lac A, and lac I. The operon is activated by the presence of lactose. The lac Z, lac Y, lac A produce beta galactosidase, lactose permease, and thiogalactoside transacetylase enzymes.

Permease enzyme allows lactose to come into the cell, and beta galactosidase hydrolyzes lactose to glucose and galactose. Transacetylase is used to functionalize substrates. If a more preferable substrate is present or lactose is absent, lac I gets activated. This produces an allolactose binding protein. In the presence of allolctose, the repressor protein molecules bind to allolactose molecules. This allows the transcription to continue without getting disturbed. In the absence of lactose, this protein binds to the promoter region (control unit) of the lac operon blocking and stopping gene transcription. When this happens no lactose permease or beta galactosidase are produced. Therefore, lactose catabolism is stopped.

Trp Operon

Trp operon is also a cluster of genes controlled by a single promoter. This operon contains all the genes required for Trp synthesis. Tryptophan commonly abbreviated as Trp is an unusual amino acid. The operon consists of trp E, trp D, trp C, trp B, and trp A, which collectively code tryptophan synthetase; the enzyme which produces tryptophan.

The trp operon also contains trp R that produces a repressor when needed. In the presence of tryptophan this operon stays deactivated because the repressor changes its conformation to active form and is bound to the promoter region. In the absence of tryptophan, the repressor protein gets released from the promoter region or is in the inactive conformation, which cannot bind to the promoter region, and thereby transcription of the genes is initiated making tryptophan, as a result. Unlike the lac operon this operon is deactivated in the presence of tryptophan, this mechanism is referred to as “negative repressive feedback mechanism”.

What is the difference between Lac operon and Trp operon?

• Lac operon is involved with the catabolic process of a sugar, but Trp operon is involved in the anabolic process of an amino acid.

• Lac operon gets activated in the presence of lactose, but Trp operon gets deactivated in the presence of tryptophan.

• Lac operon consists of three structural genes and a repressor gene, but Trp operon consists of five structural genes and a repressor gene.